Reproduction Abstracts

Dynamic epigenetic reprogramming occurs during mammalian germ cell development, whereas the targets of this process including DNA methylation/demethylation remain poorly understood. Here, we examined genome-wide methylation profiles in developing and fully developed germ cells of mice using whole-genome bisulfite sequencing (WGBS). We scaled down the construction and analysis to nanogram quantities of DNA by generating a new WGBS library, termed the post-bisulfite adapter tagg...

Introduction: Genome-wide demethylation reprograms the genome after fertilization and re-establishes totipotency: 5-methylcytosine (5 mC) in the paternal pronucleus is rapidly converted to 5-hydroxymethylcytosine (5 hmC) by the dioxygenase 10–11 translocation (TET) 3, while 5 mC in the maternal pronucleus is protected by the binding of developmental pluripotency-associated (DPPA) 3 to histone H3 dimethyl Lys9 (H3K9me2). This process is essential for normal development, be...